师资名录

陈俊升

助理研究员

 

邮箱:js.chan@sjtu.edu.cn

陈俊升,博士,助理研究员。微生物与生化药学及生物技术药物方向。
教育科研经历:
2016/06至今,BEAT365唯一官网,助理研究员
2013/11-2016/05,BEAT365唯一官网,细胞工程及抗体药物教育部工程技术中心,博士后,合作导师:朱建伟教授
2007/09-2013/06,上海医药工业研究院,硕士、博士,导师:陈代杰教授
学科方向:微生物生化药学及生物技术药物
1. 微生物源新药发现与机制研究
2. 治疗性抗体及重组蛋白药物研究
3. 微生物药物及生物技术药物制备工艺研究
(1) Ge, Y.;Wang, Z.;Lu, Y.;Rong, F.;Hao, B.;Li, Z.;Chen, J.;Wang, Y. Synergistic Metabolism Intervention and Immune Activation for Antitumor Therapy by Biomineralized Lactate Oxidase. Chemical Engineering Journal 2024, 497, 154378. https://doi.org/10.1016/j.cej.2024.154378.
(2) Ge, Y.;Rong, F.;Lu, Y.;Wang, Z.;Liu, J.;Xu, F.;Chen, J.;Li, W.;Wang, Y. Glucose Oxidase Driven Hydrogen Sulfide-Releasing Nanocascade for Diabetic Infection Treatment. Nano Lett. 2023, 23 (14), 6610–6618. https://doi.org/10.1021/acs.nanolett.3c01771.
(3) Hao, S.;Xu, S.;Li, L.;Li, Y.;Zhao, M.;Chen, J.;Zhu, S.;Xie, Y.;Jiang, H.;Zhu, J.;Wu, M. Tumour Inhibitory Activity on Pancreatic Cancer by Bispecific Nanobody Targeting PD-L1 and CXCR4. BMC Cancer 2022, 22 (1), 1092. https://doi.org/10.1186/s12885-022-10165-7.
(4) 张静;张婕雨;惠子;朱建伟;陈俊升. 断裂内含肽介导的抗体铰链区氨基酸序列的还原剂非依赖型反式剪接. 中国医药工业杂志 2022, 53 (10), 1424–1431. https://doi.org/10.16522/j.cnki.cjph.2022.10.007.
(5) Li, C.;Ge, M.;Chen, D.;Sun, T.;Jiang, H.;Xie, Y.;Lu, H.;Zhang, B.;Han, L.;Chen, J.;Zhu, J. RPL21 siRNA Blocks Proliferation in Pancreatic Cancer Cells by Inhibiting DNA Replication and Inducing G1 Arrest and Apoptosis. Front. Oncol. 2020, 10. https://doi.org/10.3389/fonc.2020.01730.
(6) 陈浩;曹津;张静;朱建伟;陈俊升. 断裂内含肽C片段在大肠埃希菌表达系统中酶解稳定性的改良. zgykdx 2020, 51 (3), 340–348. https://doi.org/10.11665/j.issn.1000-5048.20200312.
(7) 曹津;陈浩;张静;朱建伟;陈俊升. C端断裂型内含肽Npu DnaE介导的抗体片段及免疫毒素在大肠埃希菌中的可溶性表达与纯化. 中国药科大学学报 2020, 51 (2), 213–222. https://doi.org/10.11665/j.issn.1000-5048.20200213.
(8) 陈俊升;陈代杰;路慧丽. 传染病背后的科学——从“N个第一”的诞生到传染病的防治. 中国抗生素杂志 2020, 45 (04), 315–346.
(9) 窦同璐;陈浩;曹津;陈俊升;朱建伟. 断裂内含肽在含有抗体铰链区氨基酸序列的非天然外显肽中的剪接. 中国药科大学学报 2019, 50 (5), 606–613. https://doi.org/10.11665/j.issn.1000-5048.20190515.
(10) Luo, H.;Hu, L.;Ma, B.;Zhao, M.;Luo, M.;Deng, Q.;Deng, S.;Ye, H.;Lin, T.;Chen, J.;Wang, T.;Zhu, J.;Lu, H. Molecular Dynamics Based Improvement of the Solubilizing Self-Cleavable Tag Zbasic-ΔI-CM Application in the Preparation of Recombinant Proteins in Escherichia Coli. Biochemical and Biophysical Research Communications 2019, 513 (2), 412–418. https://doi.org/10.1016/j.bbrc.2019.03.205.
(11) Wang, J.;Han, L.;Chen, J.;Xie, Y.;Jiang, H.;Zhu, J. Reduction of Non-Specific Toxicity of Immunotoxin by Intein Mediated Reconstitution on Target Cells. International Immunopharmacology 2019, 66, 288–295. https://doi.org/10.1016/j.intimp.2018.11.039.
(12) Xu, Y.;Zhang, L.;Ma, B.;Hu, L.;Lu, H.;Dou, T.;Chen, J.;Zhu, J. Intermolecular Disulfide Bonds between Unpaired Cysteines Retard the C-Terminal Trans-Cleavage of Npu DnaE. Enzyme and Microbial Technology 2018, 118, 6–12. https://doi.org/10.1016/j.enzmictec.2018.06.013.
(13) 许嫣然;张蕾;窦同璐;陈俊升;朱建伟. 大肠埃希菌内锌离子对断裂内含肽Npu DnaE 自剪接作用的抑制效应. 中国医药工业杂志 2018, 49 (05), 608. https://doi.org/10.16522/j.cnki.cjph.2018.05.011.
(14) Han, L.;Chen, J.;Ding, K.;Zong, H.;Xie, Y.;Jiang, H.;Zhang, B.;Lu, H.;Yin, W.;Gilly, J.;Zhu, J. Efficient Generation of Bispecific IgG Antibodies by Split Intein Mediated Protein Trans-Splicing System. Sci Rep 2017, 7 (1), 8360. https://doi.org/10.1038/s41598-017-08641-3.
(15) Chen, H.;Li, N.;Xie, Y.;Jiang, H.;Yang, X.;Cagliero, C.;Shi, S.;Zhu, C.;Luo, H.;Chen, J.;Zhang, L.;Zhao, M.;Feng, L.;Lu, H.;Zhu, J. Purification of Inclusion Bodies Using PEG Precipitation under Denaturing Conditions to Produce Recombinant Therapeutic Proteins from Escherichia Coli. Applied Microbiology and Biotechnology 2017, 101 (13), 5267–5278. https://doi.org/10.1007/s00253-017-8265-x.
(16) Chen, H.;Li, N.;Xie, Y.;Jiang, H.;Yang, X.;Cagliero, C.;Shi, S.;Zhu, C.;Luo, H.;Chen, J.;Zhang, L.;Zhao, M.;Feng, L.;Lu, H.;Zhu, J. Purification of Inclusion Bodies Using PEG Precipitation under Denaturing Conditions to Produce Recombinant Therapeutic Proteins from Escherichia Coli. Appl Microbiol Biotechnol 2017, 101 (13), 5267–5278. https://doi.org/10.1007/s00253-017-8265-x.
(17) Ding, K.;Han, L.;Zong, H.;Chen, J.;Zhang, B.;Zhu, J. Production Process Reproducibility and Product Quality Consistency of Transient Gene Expression in HEK293 Cells with Anti-PD1 Antibody as the Model Protein. Appl Microbiol Biotechnol 2017, 101 (5), 1889–1898. https://doi.org/10.1007/s00253-016-7973-y.
(18) Shi, S.;Chen, H.;Jiang, H.;Xie, Y.;Zhang, L.;Li, N.;Zhu, C.;Chen, J.;Luo, H.;Wang, J.;Feng, L.;Lu, H.;Zhu, J. A Novel Self-Cleavable Tag Zbasic–∆I-CM and Its Application in the Soluble Expression of Recombinant Human Interleukin-15 in Escherichia Coli. Appl Microbiol Biotechnol 2017, 101 (3), 1133–1142. https://doi.org/10.1007/s00253-016-7848-2.
(19) Han, J.;Chen, J.;Shao, L.;Zhang, J.;Dong, X.;Liu, P.;Chen, D. Production of the Ramoplanin Activity Analogue by Double Gene Inactivation. PLOS ONE 2016, 11 (5), e0154121. https://doi.org/10.1371/journal.pone.0154121.
(20) Chen, J.-S.;Su, M.;Shao, L.;Wang, Y.-X.;Lin, H.-M.;Chen, D.-J. Investigation of Halogenation during the Biosynthesis of Ramoplanin in Actinoplanes Sp. ATCC33076. Appl Microbiol Biotechnol 2016, 100 (1), 289–298. https://doi.org/10.1007/s00253-015-7014-2.
(1) 陈俊升;陈灼湖;刘炳珠;刘茂花;林小敏;郑毅伟 ;一种淡紫灰链霉菌海南变种菌株及利用其制备中生菌素制品的方法, 2023-03-28, 中国, ZL202111532073.1
(2) 陈俊升;陈灼湖;温锦奋;刘炳珠;朱建伟;赵立平 ;一种中生菌素母药清洁生产方法, 2017-3-23,中国, ZL201710176413
(3) 陈俊升;朱建伟;韩雷;丁凯;谢跃庆;江华;路慧丽;张宝红;张蕾 ;基于内含肽的药用重组蛋白的合成方法, 2016-2-23, 中国, CN201610099711.8
(4) 陈俊升;陈灼湖;温锦奋;刘炳珠;朱建伟;赵立平 ;一种中生菌素发酵连续补料生产装置及其生产方法, 2019-4-9, 中国, ZL201710037400.3
(5) 韩雷;朱建伟;陈俊升;丁凯;谢跃庆;江华;路慧丽;张宝红;张蕾 ;二价双特异性抗体杂交蛋白的表达和制备方法, 2016-2-23, 中国, ZL201610100217.9
(6) 朱建伟;王静;韩雷;陈俊升;谢跃庆;江华;POLYPEPTIDE COMPOSITION, 2019-9-27, WIPO, WOCN19108588
(7) Lei Han;Jianwei Zhu;Junsheng Chen;Kai Ding;Yueqing Xie;Hua Jiang;Huili Lu;Baohong Zhang;Lei Zhang ;Bivalent bispecific antibody hybrid protein expression and preparation methods, 2016-12-16, 美国, US11535674B2
(8) HAN Lei;ZHU Jianwei;CHEN Junsheng;DING Kai;XIE Yueqing;JIANG Hua;LU Huili;ZHANG Baohong;ZHANG Lei;BIVALENT BISPECIFIC ANTIBODY HYBRID PROTEIN EXPRESSION AND PREPARATION METHODS, 2020-4-22, 欧洲, EP3418305A4
(9) 朱建伟;韩雷;陈俊升;丁凯;谢跃庆;江华;路慧丽;张宝红;张蕾 ;多价多特异性抗体及免疫杂合蛋白的表达和制备方法, 2016-2-23, 中国, ZL201610099680.6
(10) ZHU Jianwei;WANG Jing;CHEN Junsheng;XIE Yueqing;JIANG Hua;ZONG Huifang;HAN Lei ;POLYPEPTIDE COMBINATION USED FOR TUMOUR IMMUNOTHERAPY, AND PREPARATION METHOD THEREFOR, 2020-4-20, WIPO, WOCN20085736
(11) 陈俊升;邱远望;邱家亮;陈志鑫;蔡少达;一种微生物组合物及其应用,2024-06-06,中国,ZL202410732502.7
(12) 陈俊升;陈灼湖;刘炳珠;刘茂花;林小敏;郑毅伟;一种菌丝体或菌体及其应用、及利用其选育菌株的方法,2021-12-14,中国,ZL202111532064.2
(13) 朱建伟;王静;陈俊升;谢跃庆;江华;宗会芳;韩雷;一种用于肿瘤免疫治疗的多肽组合及其制备方法,2019-04-22,中国,ZL202080030426.5
(14) 朱建伟;王静;韩雷;陈俊升;谢跃庆;江华;一种多肽组合物,2019-09-27,中国,ZL201980064610.9
(15) 陈俊升; 邱远望; 邱家亮; 陈志鑫; 蔡少达;一种短促生乳杆菌及其应用,2024-06-06,中国,ZL202410732455.6
(16) 陈俊升; 陈灼湖; 温锦奋; 刘炳珠; 朱建伟; 赵立平;一种中生菌素发酵连续补料生产装置及其生产方法,2017-01-19,中国,一种中生菌素发酵连续补料生产装置及其生产方法,ZL201710037400.3

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